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Cell line: | GL-261 |
DSMZ no.: | ACC 802 |
Species: | murine (mouse) (Mus musculus) |
Cell type: | glioblastoma |
Origin: | established as in vitro growing cell culture in the mid 1990s from the Gl261 tumor which was induced in 1939 by intracranial injection of 3-methylcholanthrene into C57BL/6 mice and subsequently maintained by serial transplantation in syngeneic mouse strain; cells were described in the literature to carry TP53 and KRAS mutations |
Reference(s): | 18129, 18130, 18131, 18204, 18213 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| DSMZ Cell Culture Data: |
Morphology: | fibroblastoid cells growing adherently in monolayers; image; image |
Medium: | 90% DMEM + 10% h.i. FBS + 2 mM glutamine |
Subculture: | seed out at ca. 1 x 106 cells/175 cm2 flask; split 1:3 - 1:4 every 5-6 days using trypsin/EDTA |
Incubation: | at 37 °C with 10% CO2 |
Doubling time: | ca. 80-100 hours |
Harvest: | cell harvest of ca. 6 x 106 cells/175 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in PCR assay |
Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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