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Cell line: | GRANTA-452 |
DSMZ no.: | ACC 713 |
Species: | human (Homo sapiens) |
Cell type: | B cell leukemia |
Origin: | derived from a case of B cell acute lymphoblastic leukemia (B-ALL) following transformation from follicular B cell non-Hodgkin lymphoma (B-NHL); described in the literature to carry a t(2;9) (p1;p13) and a t(9;14;18) (p13;q32;q21), to exhibit a biallelic deletion of CDKN2A as well as a BCL2 MBR rearrangement |
Reference(s): | 16493, 15064 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | round cells growing singly in suspension; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS |
Subculture: | seed out at ca. 1 x 106 cells/ml; maintain at ca. 0.5-1 x 106 cells/ml; split culture 1:2 to 1:4 every 2-3 days |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 48 hours |
Harvest: | maximum density at ca. 2-3 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in microbiological culture, PCR assays |
Immunology: | CD3 -, CD10 +, CD13 -, CD19 +, CD20 -, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, HLA-DR +, sm/cyIgG +, sm/cyIgM -, sm/cykappa -, sm/cylambda +; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hyperdiploid karyotype with 5% polyploidy; 49(46-50)<2n>XXY, +X, +7, +11, der(1)t(1;7)(p36;p15), der(2)t(2;4)(p21;p15.2), der(3)t(1;3)(p26;q11), der(4)t(4;6)(p11;q22), del(6)(q13), trp(8)(q22q24), t(8;22)(q24;q11), der(9)t(2;9)(p13;p15.2), der(9)t(9;14)(p13;q22)t(14;18)(q32;q21), der(14)t(9;14)(p21;q22), t(16;19)(p13;p13), der(18)t(14;18)(q32;q21), der(20)t(9;20)(p21;p13)ins(9;?)(p21.2;?); carries IGH/BCL2 and IGL/MYC rearrangements, together with a complex series of rearrangements effecting deletion of CDKN2 (homozygous) and PAX5 (hemizygous); consistent with published karyotype data |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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