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| Cell line: | H-1184 |
| DSMZ no.: | ACC 502 |
| Species: | human (Homo sapiens) |
| Cell type: | small cell lung carcinoma |
| Origin: | established in 1985 from the lymph node from a 42-year-old white man with metastatic small cell lung carcinoma prior to treatment; corresponds to NCI-H1184; matched B-lymphoblastoid cell line is available (NCI-BL1184, DSMZ ACC 896) |
| Reference(s): | 14421, 14849 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | small epitheloid cells growing adherently in monolayers or in suspension as clusters; image; image; image |
| Medium: | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture: | seed out at ca. 1-2 x 106 cells/cm2 in a 80 cm2 flask; split saturated culture 1:2 to 1:4 every 5-8 days using trypsin/EDTA; after recovery change medium sparingly |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 50-70 hours |
| Harvest: | maximum density of ca. 20-25 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hyperdiploid karyotype with 8% polyploidy - 57(53-59)<2n>XY/XXY, +1, +3, +5, +8, +9, -10, -10, +11, +12, -14, +16, +20, +22, +6mar, add(1)(p34), add(1)(p11), add(3)(p14), add(3)(q21-22), add(5)(p11), add(5)(q11), add(8)(q21), add(11)(q25), add(12)(q24)x1-2, der(12)t(11;12)(q11;q24), add(13)(p11), del(?13)(q12q21), del(16)(p12p13), der(?22)t(14;22)(q21;q13) |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
