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| Cell line: | H-2171 |
| DSMZ no.: | ACC 544 |
| Species: | human (Homo sapiens) |
| Cell type: | small cell lung carcinoma |
| Origin: | derived from the pleural effusion of a 50-year-old white male with extensive small cell lung carcinoma after chemotherapy in 1989; corresponds to NCI-H2171; listed in the CCLE (ref 18232) with extensive genome and protein analyses |
| Reference(s): | 14849, 15046, 14567, 18232, 40405 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | large round cells growing singly or in clumps in suspension; image; image |
| Medium: | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture: | split saturated culture 1:2 to 1:3 every 2-4 days; seed out at ca. 1.0 x 106 cells/ml; maintain at 0.7-2.0 x 106 cells/ml; after recovery the growing is slow |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 35-60 hours |
| Harvest: | maximum density at 3.0 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hypodiploid karyotype with 8% polyploidy - 42-44<2n>XY, -4, -5, -8, -10, -11, -13, -15, -17, -19, +20, +4-5mar, add(1)(p36), der(2)ins(2;?)(p2?;?)t(2;2)(q37;p24), add(3)(p11), i(3q), del(6)(q25q26), del(9)(p11), der(12)add(12)(p11)del(12)(q24), add(14)(p11), i(15q), add(18)(p11) |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
