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Cell line: | HC-11 |
DSMZ no.: | ACC 780 |
Species: | murine (mouse) (Mus musculus) |
Cell type: | normal mammary epithelial |
Origin: | derived in 1987 as a prolactin-responsive subclone of the COMMA-1D cell line which was established from normal mammary epithelial cells of an adult female BALB/c mouse |
Reference(s): | 24247 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| DSMZ Cell Culture Data: |
Morphology: | epithelioid adherent cells growing as monolayer |
Medium: | 90% RPMI 1640 + 10% h.i. FBS + 5 µg/ml insulin (bovine or human) + 10 ng/ml EGF + 4 mM L-glutamine |
Subculture: | seed out at ca. 0.3-1 x 106 cells/25 cm2 flask; split confluent culture 1:2 - 1:4 every 2-4 days using trypsin/EDTA for about 10 min |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 50-80 hours |
Harvest: | cell harvest of ca. 6 x 106 cells/80 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in PCR assay |
Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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