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Cell line: | HCT-116 |
DSMZ no.: | ACC 581 |
Species: | human (Homo sapiens) |
Cell type: | colon carcinoma |
Origin: | established from the primary colon carcinoma of an adult man; cells were described in the literature to carry a RAS mutation in codon 13 and to be tumorgenic in nude mice |
Reference(s): | 14406, 14405 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| DSMZ Cell Culture Data: |
Morphology: | epithelial-like small cells growing as monolayer; image; image; image |
Medium: | 90% McCoy's 5A + 10% h.i. FBS + 2 mM L-glutamine (alternatively DMEM with high glucose) |
Subculture: | seed out at ca. 1 x 106 cells/80 cm2; split saturated culture 1:3 to 1:6 about 2-3 x per week using trypsin/EDTA; in confluent cultures with high densities, cells may detach spontaneously |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 25-40 hours |
Harvest: | cell harvest of ca. 10-20 x 106 cells/80 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in microbiological culture, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human near-diploid karyotype with 3% polyploidy - 45(44-46)<2n>X/XY, add(10)(q24-26), der(16)t(8;16)(q13;p13), der(18)t(17;18)(q13-21;p11.3) - resembles published karyotypes |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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