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| Cell line: | HEK293T-SLC35A1-KO |
| DSMZ no.: | ACC 988 |
| Species: | human (Homo sapiens) |
| Cell type: | embryonal kidney |
| Origin: | derivative of 293T (HEK293T), established in 2021 by CRISPR/Cas9-mediated modification of the CMP-sialic acid transporter-encoding gene SLC35A1 resulting in decreased/absent levels of the protein. The cells are described to harbour a functional knockout of the SLC35A1 gene leading to increased susceptibility to transduction with adeno-associated virus 9. The cells are therefore suited for AAV9 transduction potency assays |
| Reference(s): | 40955 |
| Biosafety level: | 1, GMO-S1 |
| Risk assessment: | The parental cell line 293T was established by transfection of the cells with sheared DNA of adenovirus type 5. The cells contain 4 to 5 copies of the left end of the virus (12% of the viral genome) including E1a and E1b genes and one copy of the right end of the virus (10% of the genome) including the E4 gene. No active viruses are produced. The cells are classified biosafety level 1 and the genetic modification renders the cell line level S1 according to German genetic engineering act. |
| Permissions and restrictions: | A;D |
| | DSMZ Cell Culture Data: |
| Morphology: | fibroblastoid cells growing adherently as monolayer; image; image |
| Medium: | 90% DMEM (high glucose) + 10% h.i. FBS |
| Subculture: | seed out at ca. 1-2 x 106 cells/80 cm2; split semi-confluent culture 1:10 - 1:20 every 2-4 days (without using trypsin/EDTA, cells can be detached by pipetting and tapping the flask) |
| Incubation: | at 37 °C with 10% CO2 |
| Doubling time: | ca. 24 hours |
| Harvest: | cell harvest of ca. 15 x 106 cells/80 cm2 |
| Storage: | frozen with 70% DMEM, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Authenticity: | STR analysis according to the global standard ASN-0002.1 (2021) resulted in a STR profile identical to 293T (ACC 635) of the reference database. |
| Species validation: | human |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
