| | |
|---|
| Cell line: | HELA-SLC35A1-KO |
| DSMZ no.: | ACC 989 |
| Species: | human (Homo sapiens) |
| Cell type: | cervix carcinoma |
| Origin: | derivative of HELA, established in 2021 by CRISPR/Cas9-mediated modification of the CMP-sialic acid transporter-encoding gene SLC35A1 resulting in decreased/absent levels of the protein. The cells are described to harbour a functional knockout of the SLC35A1 gene leading to increased susceptibility to transduction with adeno-associated virus 9. The cells are therefore suited for AAV9 transduction potency assays |
| Reference(s): | 40955 |
| Biosafety level: | 1, GMO-S1 |
| Risk assessment: | The parental cell line HELA is infected with papilloma virus type 18 (HPV-18). The cells contain several copies as proviruses integrated into the eukaryotic genome. The integrated virus genomes are incomplete and exhibit 2-3 kb deletions of the E2-L2 region. An activation and transmission of the HPV-18 during handling of the cell line is improbable. The cells are classified biosafety level 1 and the genetic modification renders the cell line level S1 according to German genetic engineering act. |
| Permissions and restrictions: | A;D |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelial-like cells growing in monolayers; image; image; image |
| Medium: | 90% DMEM (high glucose) + 10% h.i. FBS |
| Subculture: | seed out at ca. 0.5-1 x 106 cells in a T75 flask; split confluent culture 1:10 to 1:20 every 2-4 days using trypsin/EDTA |
| Incubation: | at 37 °C with 10% CO2 |
| Doubling time: | ca. 15-20 hours |
| Harvest: | cell harvest of ca. 15 x 106 cells/75 cm2 flask |
| Storage: | frozen with 70% DMEM, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Authenticity: | STR analysis according to the global standard ASN-0002.1 (2021) resulted in a STR profile identical to HELA (ACC 57) of the reference database. |
| Species validation: | human |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
