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Cell line: | HT-1080 |
DSMZ no.: | ACC 315 |
Species: | human (Homo sapiens) |
Cell type: | fibrosarcoma |
Origin: | established from the biopsy from a fibrosarcoma of a 35-year-old man in 1972; patient did not receive any chemo- or radiotherapy |
Reference(s): | 14881 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | epithelial-like adherent cells growing as monolayers; image; image; image |
Medium: | 90% DMEM + 10% h.i. FBS |
Subculture: | seed out at ca. 1-2 x 106 cells/80 cm2; split confluent culture up to 1:10 every 3-5 days using trypsin/EDTA |
Incubation: | at 37 °C with 10% CO2 |
Doubling time: | ca. 30 hours |
Harvest: | cell harvest of ca. 25 x 106 cells/175 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | to inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human near-diploid karyotype with 24% polyploidy - 46<2n>XY, der(5)t(5;5)(p15;q32), der(11)t(10;11)(q24;q11) - sideline with -13, del(1)(p32), del(1)(q24) - 5p15 is a putative recurrent breakpoint in fibrosarcoma |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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