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| Cell line: | IARC-139 |
| DSMZ no.: | ACC 924 |
| Species: | human (Homo sapiens) |
| Cell type: | B lymphoblastoid cells |
| Origin: | B-lymphoblastoid cell line established in 1982 from normal B-cells of peripheral blood of a 19-year-old man with Burkitt lymphoma. In contrast to autologous BL-30 (DSMZ ACC 916) the IARC-139 carries a wildtype TP53 gene |
| Reference(s): | 14714, 40225, 40399, 40234 |
| Biosafety level: | 2 |
| Permissions and restrictions: | A, B |
| | DSMZ Cell Culture Data: |
| Morphology: | mostly round cells growing singly and in aggregates in suspension; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | seed out at about 0.5 x 106 cells/ml; split 1:2 to 1:3 every 2-3 days and maintain at 0.3-0.5 x 106 cells/ml (densities above 1 x 106 cells/ml will induce apoptosis) |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | 48hrs |
| Harvest: | maximal density of ca. 1 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Immunology: | CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD38 -, CD80 +, CD138 -, HLA-DR +, cyIgG -, cyIgM +, cykappa +, cylambda -; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database, matching the autologous cell line BL-30 |
| Molec. Genetics: | wildtype TP53 was verified by sequencing |
| Viruses: | PCR: EBV + (the cell line contains an EBV strain that differs from the published B95-8-EBV strain), HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
