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Cell line: | IARC-174 |
DSMZ no.: | ACC 925 |
Species: | human (Homo sapiens) |
Cell type: | B lymphoblastoid cells |
Origin: | B95-8-EBV-transformed cell line established in 1984 from normal B-cells of peripheral blood of a 54-year-old woman with sporadic Burkitt lymphoma in relapse; an autologous Burkitt lymphoma cell line is available (BL-36, DSMZ ACC 917) |
Reference(s): | 40251, 14714, 40399 |
Biosafety level: | 2 |
Risk assessment: | The cell line is positive for EBV (HHV-4) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 was also positive by western blot analysis in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as lytic associated with a production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is therefore classified biosafety level 2. |
Permissions and restrictions: | A, B |
| DSMZ Cell Culture Data: |
Morphology: | round to polymorph cells growing singly and in aggregates in suspension; image; image; image |
Medium: | 80% Iscove's MDM + 20% h.i. FBS + 100 µM alpha-thioglycerol |
Subculture: | seed out at about 0.5 x 106 cells/ml; split 1:2 to 1:3 every 2-3 days, maintain at 0.3-0.5 x 106 cells/ml |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 2-3 days |
Harvest: | maximal density of ca. 2 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in PCR assay |
Immunology: | CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD80 +, CD138 -, HLA-DR +, cyIgG -, cyIgM +, cykappa +, cylambda -; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database, matching the autologous cell line BL-36 |
Viruses: | PCR: EBV + (cells carry the EBV strain B95.8), HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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