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| Cell line: | IARC-290A |
| DSMZ no.: | ACC 929 |
| Species: | human (Homo sapiens) |
| Cell type: | B lymphoblastoid cells |
| Origin: | B95-8-EBV-transformed cell line established in 1982 from normal B-cells of peripheral blood of a 33-year-old woman with sporadic Burkitt lymphoma; an autologous Burkitt lymphoma cell line is available (BL-74, DSMZ ACC 922) |
| Reference(s): | 40251, 14714, 40431 |
| Biosafety level: | 2 |
| Risk assessment: | The cell line is positive for EBV (HHV-4) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 was also positive by western blot analysis in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as lytic associated with a production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is therefore classified biosafety level 2. |
| Permissions and restrictions: | A, B |
| | DSMZ Cell Culture Data: |
| Morphology: | mostly round single cells growing in suspension and partly in small clusters; image; image |
| Medium: | 80% Iscove's MDM + 20% h.i. FBS + 2 mM L-glutamine + 100 µM alpha-thioglycerol |
| Subculture: | seed out at 0.2 - 0.4 x 106 cells/ml; split 1:3 to 1:4 every 2-3 days, maintain at 0.5 - 1 x 106 cells/ml |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 50-60 hours |
| Harvest: | maximal density of ca. 1.5 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Immunology: | CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD80 +, HLA-DR +; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database, matching the autologous cell line BL-74 |
| Viruses: | PCR: EBV + (cells carry the EBV strain B95-8), HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
