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| Cell line: | ICC18 |
| DSMZ no.: | ACC 11026 |
| Species: | human (Homo sapiens) |
| Cell type: | biliary tract cancer |
| Origin: | established in 2016 from the primary untreated tumor of a woman with intraductal papillary neoplasm of the bile duct in the liver (intrahepatic cholangiocarcinoma). The cell line to carries a mutation in TP53 pY205H. Alias: MGH-ICC18 |
| Reference(s): | 41166, 41515 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelial-like cells growing adherently in islands; image; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | seed out at ca. 1 x 106 cells/T175 flask; split 1:4-1:8 every 5-7 days using trypsin/EDTA (5 minutes) |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 50-70 hours (ca. 3 days directly after thawing) |
| Harvest: | cell harvest of ca. 4-5 x 106 cells/ T175 flask |
| Storage: | frozen with 70% RPMI 1640, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database |
| Species validation: | human |
| Molec. Genetics: | homozygous TP53 Y205H mutation was confirmed by RNA-seq analysis; positive for telomerase activity (by TRAP assay) |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
