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| Cell line: | ICC4 |
| DSMZ no.: | ACC 996 |
| Species: | human (Homo sapiens) |
| Cell type: | biliary tract cancer |
| Origin: | established in 2016 from a patient derived xenograft model (PDX) from the spine metastasis of an intrahepatic cholangiocarcinoma of a 61-year-old pretreated man. The cell line is described to carry the following genetic changes: TP53 L137Q, TSPAN6 deletion, MUC4 mutations/deletions |
| Reference(s): | 41167, 41168, 41169, 41170 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | epitheloid cells growing adherently as monolayer; image; image; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | seed out at ca. 1 x 106 cells/80 cm2; split 1:5 once or twice a week using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 48-72 hours |
| Harvest: | cell harvest of ca. 10 x 106 cells/80 cm2 |
| Storage: | frozen with 70% RPMI 1640, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database |
| Species validation: | human |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
