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| Cell line: | ICC4 |
| DSMZ no.: | ACC 996 |
| Species: | human (Homo sapiens) |
| Cell type: | biliary tract cancer |
| Origin: | established in 2016 from a patient derived xenograft model (PDX) from the spine metastasis of an intrahepatic cholangiocarcinoma of a 61-year-old pretreated man. Cells carry the following genetic changes: TP53 L137Q, TSPAN6 deletion, MUC4 mutations/deletions |
| Reference(s): | 41515, 41168, 41169, 41170 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | epitheloid cells growing adherently as monolayer; image; image; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | seed out at ca. 1 x 106 cells/T75 flask; split 1:5 once or twice a week using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 48-72 hours |
| Harvest: | cell harvest of ca. 10 x 106 cells/T75 flask |
| Storage: | frozen with 70% RPMI 1640, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database |
| Species validation: | human |
| Molec. Genetics: | Three mutations of cell line ICC4 reported in Vijay et al. (2024) and listed on depmap.org were confirmed by RNA sequencing (ARID1A p.G410Ter, BAP1 p.I586NfsTer54, TP53 p.L137Q). Positive for telomerase activity (by TRAP assay) |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
