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| Cell line: | ICC8 |
| DSMZ no.: | ACC 999 |
| Species: | human (Homo sapiens) |
| Cell type: | biliary tract cancer |
| Origin: | established in 2016 from a patient derived xenograft model (PDX) of the primary untreated tumor of a 57-year-old man suffering from intrahepatic cholangiocarcinoma. Cells carry AKT1 E17K and BRAF V600E mutations and are described to exhibit fusion genes HIF1A::PCNX4, USP48::HSPG2, and EZR::RSPH3 |
| Reference(s): | 41515, 41168 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | very big epitheloid cells growing adherently as monolayer; large vacuoles develop in many cells during cultivation; image; image; image; image; image; image |
| Medium: | 85% RPMI 1640 + 15% h.i. FBS |
| Subculture: | seed out at ca. 1 x 106 cells/T75 flask; split 1:4 to 1:5 every 4-6 days using trypsin/EDTA; after thawing cell viability drops to about 60-70% |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 40-60 hours |
| Harvest: | cell harvest of ca. 5 x 106 cells/T75 flask |
| Storage: | frozen with 70% RPMI 1640, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database |
| Species validation: | human |
| Molec. Genetics: | mutations of AKT1 E17K (homozygous) and BRAF V600E (heterozygous) were verified by RNA-seq analysis; positive for telomerase activity (by TRAP assay) |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
