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| Cell line: | IGR-37 |
| DSMZ no.: | ACC 237 |
| Species: | human (Homo sapiens) |
| Cell type: | melanoma |
| Origin: | established from the lymph node metastasis (groin) of a 26-year-old man with malignant melanoma (primary tumor histology: SSM level IV); cell line IGR-39 from the primary tumor is available (DSMZ ACC 239); described as strong tumorigenic in nude mice |
| Reference(s): | 14363, 40558 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelial-like cells growing as monolayers; image; image |
| Medium: | 85% DMEM + 15% h.i. FBS |
| Subculture: | seed out at ca. 2.5 x 106 cells/75 cm2; split confluent culture 1:2 every 7-14 days using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca.48-72 hours |
| Harvest: | cell harvest of about 2-3 x 105/cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | initial contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization and continuously in PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database, matching the profile of sister cell line IGR-39 |
| Cytogenetics: | human flat-moded hypertriploid karyotype with 11% polyploidy - 78(67-78)<3n>XXY, +1, +3, +5, +7, +7, +8, +9, -10, -12, -13, -14, -15, -17, +18, +20, +20, +2mar, der(1)t(1;?21)(p13;q11)x2, del(9)(p13p21), der(10)t(9;10)(q11/21;q23/25), i(13q)x1-2, der(16)t(1;16)(p11/13;q21/23)x2, der(19)t(17;19)(q12;p13)x1-2 - rearrangements at 1p12/22, 19p13 and +7 recur in malignant melanoma |
| Molec. Genetics: | Genomic sequencing and/or ARMS assay show that the cell line carries the BRAF V600E mutation (mu/wt), but no NRAS Q61 mutation |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
