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| Cell line: | INA-6 |
| DSMZ no.: | ACC 862 |
| Species: | human (Homo sapiens) |
| Cell type: | multiple myeloma |
| Origin: | established from the pleural effusion of an 80-year old patient with plasma cell leukemia in 1988. Cell line is IL-6 dependent and was described in the literature to carry an activating mutation of the N-RAS gene and del(1)(p22), del(7)(p13) and 14q+: t(11;14)(q13;q32) translocation |
| Reference(s): | 18191, 18192, 18193, 18194 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | round cells of different sizes, growing singly or in small clumps in suspension; image; image; image |
| Medium: | 80% RPMI 1640 + 20% FBS + 10 ng/ml IL-6 |
| Subculture: | seed out 0.5-1.0 x 106 cells/well in a 24-well plate or at 0.5 x 106 cells/ml in a small culture flask; maintain at 0.5-1.5 x 106 cells/ml, split 1:2 to 1:4 every 2-3 days. After thawing growth may be delayed |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | about 30-50 hours |
| Harvest: | maximal density of ca. 2 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Immunology: | CD3-, CD10-, CD13-, CD19-, CD20-, CD34-, CD37-, CD38-, CD80-, CD138(+), HLA-DR-, cyCD79a-, cIgG-, cIgM-, cIg-kappa+, cIg-lambda-, sIgG-, sIgM-, sIg-kappa-, sIg-lambda-; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
