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| Cell line: | IPEC-1 |
| DSMZ no.: | ACC 705 |
| Species: | porcine (pig) (Sus scrofa) |
| Cell type: | intestine (jejunum/ileum) |
| Origin: | established from normal intestinal epithelium cells isolated from both jejunum and ileum of a neonatal pig |
| Reference(s): | 16042, 16044, 16043 |
| Biosafety level: | 1 |
| Risk assessment: | The cell line carries Porcine type-C oncovirus (PCOV) sequences and their transcripts, which were detected by PCR and RT-PCR, respectively. We did not determine the infection mode of these viruses and cannot exclude the secretion of the viruses. In Germany, these viruses are classified BSL 1 regarding men and BSL 2 relating to animals (TRBA 462). However, the German Central Committee on Biological Safety (ZKBS) classified these viruses and infected cell lines BSL 2 when they are used for genetically modifying applications (Ref. 6790-10-65). |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | epitheloid cells growing adherently in monolayers and clusters; image; image; image; image; image; image |
| Medium: | 90% mixture of Ham's F12 + DMEM (at 1:1) + 10% h.i. FBS |
| Subculture: | seed out at ca. 1-2 x 106 cells/80 cm2 flask; split almost confluent culture 1:4 once or twice a week using trypsin/EDTA for about 10 minutes |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 30 hours |
| Harvest: | cell harvest of ca. 4-5 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Sus scrofa species. |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
