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Cell line: | JIH-5 |
DSMZ no.: | ACC 788 |
Species: | human (Homo sapiens) |
Cell type: | acute biphenotypic leukemia |
Origin: | established in 2009 from leukemic bone marrow cells of a 21-year old woman with mixed-phenotype acute leukemia (MPAL) at second relapse; cells express typical antigens of myeloid as well as B-lymphoid lineages; cells were described in the literature to express EP300-ZNF384 fusion gene |
Reference(s): | 18132 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| DSMZ Cell Culture Data: |
Morphology: | single round cells growing in suspension; image; image |
Medium: | 80% Iscove's MDM + 20% h.i. FBS |
Subculture: | seed out at ca. 1.5 x 106 cells/well in 24 well plates, subsequently cells may be grown in flasks, optimal split ratio 1:1.5 - 1:2 every 5-7 days, maintain between 1 and 2 x 106 cells/ml |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 5-7 days |
Harvest: | maximum density at ca. 2.5 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in PCR assay |
Immunology: | CD3 -, CD10 -, CD13 +, CD15 +, CD19 +, CD20 -, CD21 -, CD34 -, CD37 -, CD38 +, CD138 -, HLA-DR +; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Molec. Genetics: | expression of fusion genes EP300-ZNF384 and CHD4-EP300 confirmed by RT-PCR |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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