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| Cell line: | JJN-3 |
| DSMZ no.: | ACC 541 |
| Species: | human (Homo sapiens) |
| Cell type: | plasma cell leukemia |
| Origin: | established from the bone marrow of a 57-year-old woman with plasma cell leukemia (IgA1kappa) at diagnosis in 1987; JJN-3 is a subclone of the parental cell line JJN-1; plasma cell leukemia is related to multiple myeloma |
| Reference(s): | 14486, 14635 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | single round to oval cells showing a morphological range from mononuclear to occasionally multinucleated cells growing in suspension; image; image |
| Medium: | 40% DMEM + 40% Iscove's MDM + 20% h.i. FBS |
| Subculture: | seed out at ca. 0.5-1.0 x 106 cells/ml; split saturated culture 1:2 to 1:4 every 2-3 days |
| Incubation: | at 37 °C with 10% CO2 |
| Doubling time: | ca.40-50 hours |
| Harvest: | cell harvest of ca. 2 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | CD3-, CD10-, CD13-, CD19-, CD20-, CD34-, CD37-, CD38-, CD80-, CD138(+), HLA-DR(+),
cIgG+, cIgM-, cyCD79a-, cIg-kappa+, cIg-lambda-, sIgG+, sIgM-, sIg-kappa+, sIg-lambda-; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human flat-moded hypotriploid karyotype with 9% polyploidy - 58-67<3n>XX, +1, -2, +5, +8, +8, -9, -11, -12, -13, -15, -17, +20, add(1)(p22), der(1)t(1;?3)(q41;p26)x2, add(3)(p26), add(5)(p15)x1-2, i(5p), del(6)(q13),del(7)(q32), der(7)t(7;11)(q36;q13), add(8)(p1?)x2, t(12;19)(p13;q13), der(14)add(14)(p11)t(14;16)(q32;q23), der(14)t(14;16)(q32;q23), der(16)t(14;16)(q32;q23)x2, del(22)(q12) - carries two copies of t(14;16) associated with c-MAF activation - resembles published karyotype |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
