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| Cell line: | JK-1 |
| DSMZ no.: | ACC 347 |
| Species: | human (Homo sapiens) |
| Cell type: | chronic myeloid leukemia in blast crisis |
| Origin: | established from the biopsy material of the shoulder tumor of a 62-year-old man with chronic myeloid leukemia (CML) in erythroid blast crisis in 1987; described in the literature to produce hemoglobin; cells are described in the literature to carry t(9;22) leading to BCR-ABL1 e13-a2 (b2-a2) fusion gene |
| Reference(s): | 14824, 14479, 14488 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | round single cells growing in suspension (cell pellet shows reddish color hemoglobin); image; image; image |
| Medium: | 80% RPMI 1640 + 10% h.i. FBS |
| Subculture: | seed out at ca. 0.5 x 106 cells/ml in 24-well-plates; maintain at 0.3-1.0 x 106 cells/ml; split 1:2 to 1:3 every 2-3 days; it is recommended to culture the cells in 24-well-plates continuously |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 50 hours |
| Harvest: | maximal density of ca. 1.2 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | CD3-, CD13(+), CD14-, CD15-, CD19-, CD33+,
CD34(+), CD41-, CD42-, CD71+, CD235+; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hyperdiploid karyotype with significant polyploidy - 48(45-48)<2n>XY, +8, +22, t(1;7)(q10;q10), der(9)t(9;22)(q34;q11), der(22)t(9;22)(q34;q11)x2 - carries two Ph - resembles published karyotype |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
