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| Cell line: | JTC-15 |
| DSMZ no.: | ACC 329 |
| Species: | rat (Rattus norvegicus) |
| Cell type: | hepatoma |
| Origin: | established in 1963 from the ascites of a rat with hepatoma induced with 4-dimethylaminoazobenzene |
| Reference(s): | 15002, 14621 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent, epithelial cells growing in monolayers; image; image |
| Medium: | 90% DMEM + 10% h.i. FBS |
| Subculture: | split confluent culture 1:5 to 1:8 every 4-5 days using trypsin/EDTA; seed out at ca. 1-2 x 106 cells/175 cm2 |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 30 hours |
| Harvest: | cell harvest of ca. 30 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species. |
| Cytogenetics: | rat hyperdiploid/hypotriploid karyotype with 33% polyploidy - 51(51-58)<2n> - secondary constriction marker present - resembles published karyotype |
| Viruses: | ELISA: reverse transcriptase positive; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
