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| Cell line: | JTC-27 |
| DSMZ no.: | ACC 330 |
| Species: | rat (Rattus norvegicus) |
| Cell type: | hepatoma |
| Origin: | established from the transplantable rat ascites hepatoma AH-601 induced by feeding with aminoazotoluene; the hepatoma was passaged by intraperitoneal transplantation though JAR-2 rats |
| Reference(s): | 14621, 15002 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent, epithelial-like cells growing in monolayers; image; image |
| Medium: | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture: | split confluent culture 1:6 to 1:8 twice a week using trypsin/EDTA; seed out at ca. 2 x 104 cells/cm2 |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 37 hours |
| Harvest: | cell harvest of maximally 2.5-3 x 105 cells/cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI,microbiological culture, RNA hybridization assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species. |
| Cytogenetics: | rat hyperdiploid karyotype with 15% polyploidy - 47(45-49)<2n> |
| Viruses: | ELISA: reverse transcriptase negative; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
