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Cell line: | JVE-528 |
DSMZ no.: | ACC 826 |
Species: | human (Homo sapiens) |
Cell type: | colon carcinoma |
Origin: | established from the primary tissue of the ascending colon of a 57-year-old woman with malignant adenocarcinoma (Dukes stage B1); cells were described in literature to carry mutations in the KRAS, TP53 and APC genes
and methylation of the MGMT promoter |
Reference(s): | 18128, 18149 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| DSMZ Cell Culture Data: |
Morphology: | epithelioid cells growing adherently in monolayers and in densely packed islets, sometimes in multilayered clusters; note: freshly passaged cells need 3-4 days to adhere; image; image; image |
Medium: | 80% RPMI 1640 + 20% h.i. FBS |
Subculture: | seed out at ca. 2-3 x 106 cells/25 cm2;
split confluent culture 1:2 once a week using trypsin/EDTA; however cells may need several days to adhere after thawing and trypsination; we recommend to use always a new culture flask after trypsination |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 4 days |
Harvest: | cell harvest of 12-14 x 106 cells/80 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in PCR assay |
Immunology: | to inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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