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| Cell line: | JVM-2 |
| DSMZ no.: | ACC 12 |
| Species: | human (Homo sapiens) |
| Cell type: | chronic B cell leukemia |
| Origin: | established from the peripheral blood of a 63-year-old woman with B-prolymphocytic leukemia (B-PLL) at diagnosis; recent molecular biological characterization assigned this cell line to the entity "mantle cell lymphoma" (MCL); cell line was established by EBV-transformation during treatment with phorbol ester TPA; cells were described in the literature to have immunoglobulin light chain restriction (lambda) and to carry t(11;14) leading to IGH-CCND1 (BCL1) fusion gene and CCND1 activation. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and RNA-Seq) |
| Reference(s): | 14760, 14759, 15229, 18187 |
| Biosafety level: | 1 |
| Risk assessment: | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. According to a recommendation of the German Central Committee for Biological Safety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used in genetic engineering projects. |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | lymphoblastoid, round to polygonal cells growing in clusters in suspension; image; image; image; image; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | seed out at ca. 0.5 x 106 cells/ml; maintain at 0.1-0.5 x 106 cells/ml; cells might grow slowly after thawing; in log-phase split 1:2 to 1:4 every 2-4 days |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 50-70 hours |
| Harvest: | maximal density at about 1.5 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 +, HLA-DR +, cyIgG -, cyIgM +, cykappa -, cylambda +; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human pseudodiploid karyotype with 1.8% polyploidy - 46(42-46)<2n>XX, t(1;15)(q32.3;q22.1), der(8)t(3;8)(q13;p21), t(11;14)(q13;q32), dup(16)(p11p13.2) - resembles published karyotype - carries t(11;14) associated with CCND1 (cyclin D1) activation |
| Molec. Genetics: | expression of fusion gene IGH-CCND1 (BCL1) confirmed by RT-PCR |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
