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Cell line: | JVM-3 |
DSMZ no.: | ACC 18 |
Species: | human (Homo sapiens) |
Cell type: | chronic B cell leukemia |
Origin: | established from the peripheral blood of a 73-year-old man with B-prolymphocytic leukemia (PLL) at diagnosis; cell line was established by EBV-transformation during treatment with phorbol ester TPA; cells express mRNA of the proto-oncogenes BCL2 and BCL3. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence
and RNA-Seq) |
Reference(s): | 14760, 14895, 14759, 18187 |
Biosafety level: | 1 |
Risk assessment: | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. According to a recommendation of the German Central Committee for Biological Safety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used in genetic engineering projects. |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | lymphoblastoid cells growing singly or in clumps in suspension; image; image; image; image; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS |
Subculture: | seed out at ca. 0.5 x 106 cells/ml; maintain at 0.1-0.5 x 106 cells/ml; split saturated culture 1:3 to 1:4 every 3-4 days |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 30-40 hours |
Harvest: | maximum density at 1.0-1.5 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | initial contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, and continuously in PCR assays |
Immunology: | CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, cyCD79a +, CD80 +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human near tetraploid karyotype with clear mode - 87-93<4n>XXYY, +3, -7, -8, -10, +12, +12, -14, -19, -19, -20, -20 - appears to be derivate of original hyperdiploid line which exhibited trisomy 12 |
Molec. Genetics: | exome sequencing revealed a BRAF K601N mutation |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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