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Cell line: | KELLY |
DSMZ no.: | ACC 355 |
Species: | human (Homo sapiens) |
Cell type: | neuroblastoma |
Origin: | established in 1983 from an 1 year-old girl with neuroblastoma; described to carry
an ALK and a TP53 mutation and a ca. 100-fold genomic amplification of N-MYC in der(17)hsr; widely used and extensively characterized cell line; listed in the CCLE (ref 18232) |
Reference(s): | 14858, 14947, 18232 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | adherent, round to fusiform with polar neuritic processes, growing in mono- and multilayers; image; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS |
Subculture: | seed out at ca. 2-3 x 106 cells/80 cm2; split confluent culture 1:3 to 1:10 every 3-6 days using trypsin/EDTA |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 30-40 hours |
Harvest: | cell harvest of ca. 25-30 x 106 cells/80 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | original contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, and continously in PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human near-diploid karyotype with 20% polyploidy - 46-47<2n>X, -7, -8, +16, +22, +2mar, del(1)(p34), t(2;7)(p12;q11), add(4)(p15.3), add(5)(p15), add(9)(q34), add(10)(q24), add(11)(q24), add(14)(q32), ins(17;2)(q21;p24 hsr)x2 - del(1p34) and 2p24 hsr are typical of late-stage neuroblastoma - resembles published karyotype |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV +, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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