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| Cell line: | KMS-12-BM |
| DSMZ no.: | ACC 551 |
| Species: | human (Homo sapiens) |
| Cell type: | multiple myeloma |
| Origin: | established from the bone marrow of a 64-year-old woman with multiple myeloma in 1988; sister cell line of KMS-12-PE (DSMZ ACC 606). Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and RNA-Seq) |
| Reference(s): | 14817, 14486, 18187 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | mostly single cells in suspension, 1-2% giant cells; image; image; image |
| Medium: | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture: | seed out at ca. 0.5 x 106 cells/ml; split saturated culture 1:2 every 2-3 days; maintain at ca. 0.5-1.0 x 106 cells/ml; first week after thawing cultivate in 24-well-plate, initially cells grow slowly |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 60 hours |
| Harvest: | cell harvest of ca. 1.5 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | CD3-, CD10-, CD13-, CD19-, CD20-, CD34-,
CD37+, CD38(+), CD80-, CD138(+), HLA-DR-,
sIgG(+), sIgM-, cIgG-, cIgM-, sIg-kappa-,
sIg-lambda-, cIg-kappa-, cIg-lambda-; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human flat-moded hypertriploid karyotype with 3% polyploidy - 69-79<3n>XX, -X, +1, +1, -4, +5, +6, +8, +9, -10, +13, +14, -15, -16, +17, -18, +19, +20, +21, -22, +4-5mar, der(1)t(1;4)(q11;q32), del(1)(p21)x2, der(1;8)(q10;q10)x2, add(4)(q32)x1-2, der(5;17)(p10;q10)x2, add(6)(q2?5), der(9)add(9)(p24)t(9;11)(q34;q13), der(9)t(9;11)(q34;q13), der(11)t(11;14)(q13;q32)x2-3, idic(?;13)(?;q32-33)x2, der(14)t(11;14)(q13;q32), der(17)t(11;17)(q21.2;q22)t(11;14)(q13;q32)x2, add(19)(q13), add(19)(q13) - related to published karyotype - carries semi-cryptic t(11;14) with IGH-CCND1 rearrangement |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
