This online shop is using cookies to give you the best shopping experience. Thereby for example the session information or language setting are stored on your computer. Without cookies the range of the online shop's functionality is limited.
If you don't agree, please click here.
established from the peripheral blood of a 3-month-old girl with B cell precursor acute lymphoblastic leukemia (BCP-ALL) of type B-III in 1977; cells carry the t(11;19)(q23;p13) leading to the KMT2A-MLLT1 (MLL-MLLT1; MLL-ENL) fusion gene. Exome and RNA sequence data are available (see Ref 18187 and
single round rather small cells growing in suspension; image
90% RPMI 1640 + 10% h.i. FBS
seed out at ca. 1.0 x 106 cells/ml in a 12-well-plate with 20% FBS; maintain at ca. 0.5-1.5 x 106 cells/ml; split saturated culture 1:3 to 1:5 every 3 days; the viability may drop to about 10% after thawing, but the cells will recover thereafter; we recommend to centrifuge the cells in order to remove cellular debris
at 37 °C with 5% CO2
ca. 48 hours
cell harvest of ca. 2.0 x 106 cells/ml
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
initial contamination was eliminated with Baytril (enrofloxacin), then negative in microbiological culture and continuously in PCR assays