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| Cell line: | KOPN-8 |
| DSMZ no.: | ACC 552 |
| Species: | human (Homo sapiens) |
| Cell type: | B cell precursor leukemia |
| Origin: | established from the peripheral blood of a 3-month-old girl with B cell precursor acute lymphoblastic leukemia (BCP-ALL) of type B-III in 1977; cells carry the t(11;19)(q23;p13) leading to the KMT2A-MLLT1 (MLL-MLLT1; MLL-ENL) fusion gene. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence
and RNA-Seq) |
| Reference(s): | 14744, 14493, 15153, 40437, 18187 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | single round rather small cells growing in suspension; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | seed out at ca. 1.0 x 106 cells/ml in a 12-well-plate with 20% FBS; maintain at ca. 0.5-1.5 x 106 cells/ml; split saturated culture 1:3 to 1:5 every 3 days; the viability may drop to about 10% after thawing, but the cells will recover thereafter; we recommend to centrifuge the cells in order to remove cellular debris |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 48 hours |
| Harvest: | cell harvest of ca. 2.0 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | initial contamination was eliminated with Baytril (enrofloxacin), then negative in microbiological culture and continuously in PCR assays |
| Immunology: | CD3 -, CD10 +, CD13 -, CD19 +, CD34 -, CD38 +, CD80 -, CD138 -, HLA-DR +, cyIgG -, cyIgM +; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hypodiploid karyotype with 4% polyploidy - 45(42-45)<2n>XX, -14, t(8;13)(q24;q21.2), t(11;19)(q23;p13), der(13)t(13;14)(p11;q11) - carries t(11;19) effecting KMT2A-MLLT1 (MLL-MLLT1; MLL-ENL) fusion - matches published karyotype |
| Molec. Genetics: | expression of fusion gene KMT2A-MLLT1 (MLL-MLLT1; MLL-ENL) confirmed by RT-PCR |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
