| | |
|---|
| Cell line: | KYSE-510 |
| DSMZ no.: | ACC 374 |
| Species: | human (Homo sapiens) |
| Cell type: | esophageal squamous cell carcinoma |
| Origin: | established from the well-differentiated squamous cell carcinoma of the esophagus with moderate invasion of the adventitia resected from cervical esophagus of a 67-year-old woman after cisplatin and radiotherapy; described as carrying TP53 mutation and amplification of MYC, HST1, CYCLIN D1, and to induce well-differentiated tumors in nude mice |
| Reference(s): | 14646, 15006, 14954, 14955, 18232 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | epitheloid cells growing as monolayer with multilayer foci; image; image; image |
| Medium: | 90% RPMI 1640 (or mixture of RPMI 1640 + Ham's F12 at 1:1) + 10% h.i. FBS |
| Subculture: | seed out at ca. 0.5-3.0 x 106 cells/175 cm2; split confluent culture 1:5 to 1:20 every 4-8 days using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 30 hours |
| Harvest: | cell harvest of ca. 15-25 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | initial contamination was eliminated with BM-Cyclin (tiamulin & minocycline), thereafter negative in DAPI, microbiological culture, RNA hybridization, and continuously in PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hypertriploid karyotype with 8% polyploidy - 73(66-73)<3n>XXX, -X, +1, +1, -2, -2, +3, -4, +5, +6, +7, -14, -14, +19, -21, -21, +22, +9mar, add(X)(p22.2), add(1)(q21), ider(1)(q10)t(1;8)(q21;q11), add(2)(q11), del(3)(q21), del(3)(p1?), add(5)(p14), i(8p), der(8)add(8)(p11)add(8)(q24), i(9p), add(9)(p11), add(11)(p15), add(11)(q13)x2, add(14)(p11), add(15)(p11)x2, der(18)add(18)(p11)dup(18)(q11q23)add(18)(q23), der(18)add(18)(p11)add(18)(q2?3), der(18)add(18)(p11)add(8)(q2?1), del(19)(p13)x2, der(19)t(10;19)(q11;q13), add(21)(p11), del(22)(q13) |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HPV -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
