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| Cell line: | L-591 |
| DSMZ no.: | ACC 602 |
| Species: | human (Homo sapiens) |
| Cell type: | B lymphoblastoid cells (from patient with Hodgkin lymphoma) |
| Origin: | established from the pleural effusion of a 31-year-old woman with Hodgkin lymphoma (nodular sclerosis; stage IVB) in 1982 |
| Reference(s): | 14463, 15213, 40211 |
| Biosafety level: | 2 |
| Risk assessment: | The cell line is positive for EBV (HHV-4) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were also positive by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as lytic associated with a production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is thus categorized biosafety level 2. |
| Permissions and restrictions: | A, B |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | round cells growing in loosely-attached clusters; image; image; image |
| Medium: | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture: | split culture 1:2 twice a week; seed out at ca. 0.5 x 106 cells/ml in 24-well-plates; optimal growth is achieved using 24-well-plates, but culture can be adapted to grow in flasks with lower viabilities; maintain at ca. 0.5 x 106 cells/ml |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 72 hours |
| Harvest: | maximum density at ca. 2 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | CD2 +, CD3 -, CD4 -, CD8 -, CD10 -, CD19 +, CD20 -, CD21 +, CD25 +, CD30 +, CD34 -, CD80 +, CD138 -; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human near-diploid karyotype with 6% polyploidy; 46(45-48)<2n>XX, add(7)(q32), der(14)t(9;14)(p13;q32); der(14) effects rearrangement of PAX5 which (untypically for HL) is upregulated |
| Viruses: | PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
