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| Cell line: | LAT |
| DSMZ no.: | ACC 349 |
| Species: | ovine (sheep) (Ovis aries) |
| Cell type: | aortic tissue cells |
| Origin: | established from the aortic tissue cells of a lamb; cells were described in the literature to be susceptible to Visna Maedi Virus and to be free of bovine viral diarrhoea virus (BVDV) |
| Reference(s): | not published |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | fibroblastoid, growing in monolayers, forming adherent clumps upon reaching confluency; image; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | split near-confluent culture 1:5 every 4-5 days using trypsin/EDTA; seed out at ca. 1.5 x 106 cells/80 cm2; optimal density at 1.0-4.5 x 104 cells/cm2 |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | 30-50 hours |
| Harvest: | cell harvest of ca. 5 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS and 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Ovis aries species. |
| Cytogenetics: | ovine diploid karyotype with 27% polyploidy - 54<2n>XY, no consistent abnormality detected |
| Viruses: | ELISA: reverse transcriptase positive; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
