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| Cell line: | LB5848-LYMP |
| DSMZ no.: | ACC 870 |
| Species: | human (Homo sapiens) |
| Cell type: | B cell lymphoma |
| Origin: | established in 2016 from a parotid lymph node of a 75-year-old man with diffuse large B-cell lymphoma (GCB subtype, stage IB). Cells are described to exhibit an abnormal hyperdiploid and complex karyotype including BCL2 and MYC translocations (double-hit DLBCL). |
| Reference(s): | 18202 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | mostly round cells growing as single cells and in small clumps in suspension; image; image; image |
| Medium: | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture: | cells are difficult to culture; seed out at 0.25 x 106 cells in 2ml in a 24-well-plate (alternatively 0.1 x 106 cells/ml in a culture flask); maintain at 0.25-1.0 x 106 cells/ml (do not exceed 1.5 x 106 cells/ml); split 1:2 to 1:4 every 2-4 days; cell viability decreases with increasing cell density |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 50-70 hours |
| Harvest: | maximal density of ca. 1.5 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Immunology: | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD79a (+), CD80 (+), CD138 -, HLA-DR -,
sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -
; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Molec. Genetics: | expression of BCL2 and MYC confirmed by western blotting |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
