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| Cell line: | LF-CL2A |
| DSMZ no.: | ACC 195 |
| Species: | rat (Rattus norvegicus) |
| Cell type: | hepatocarcinoma |
| Origin: | a primary liver carcinoma was induced by dimethyl-aminoazobenzene (DAB) in a Wistar-Commentry rat; the hepatoma was transferred to a syngeneic rat and a cell line was established and subcloned in 1966 |
| Reference(s): | 14601, 15147, 14422 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelioid cells growing adherently as monolayer; image; image; image; image |
| Medium: | 90-95% DMEM + 5-10% h.i. FBS |
| Subculture: | split confluent culture about every 2 days using trypsin/EDTA; cells grow quickly; seed about 1 x 106 cells into a 80 cm2 flask |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 20-25 hours |
| Harvest: | cell harvest of about 30 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species. |
| Cytogenetics: | rat flat-moded near-triploid karyotype - 58-67<3n> - large submetacentric marker present in most cells |
| Viruses: | ELISA: reverse transcriptase positive; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
