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| Cell line: | LP-1 |
| DSMZ no.: | ACC 41 |
| Species: | human (Homo sapiens) |
| Cell type: | multiple myeloma |
| Origin: | established from the peripheral blood of a 56-year-old woman with multiple myeloma (IgG, EBV-negative) in leukemic transformation (refractory, terminal) in 1986. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and RNA-Seq) |
| Reference(s): | 14843, 14486, 18187 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | single, elongated cells in suspension; image; image; image; image; image; image; image; image |
| Medium: | 80-90% Iscove's MDM + 10-20% h.i. FBS |
| Subculture: | seed out at ca. 0.7 x 106 cells/well; maintain at 0.5-1.0 x 106 cells/ml; split 1:2 every 2-3 days; viability may be low initially after thawing; it is recommended to cultivate the cells in 24-well-plates during the first week and to exchange medium with dilution; cells may be partially (lightly) adherent but can be flushed off by pipetting |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | 35-80 hours (depending on cell density) |
| Harvest: | maximal density at about 1.0-1.5 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | CD3-, CD10-, CD13-, CD19-, CD20-,
CD34-, CD37-, CD38+, CD80-, CD138+, HLA-DR+, cyCD79a-, sIgG+, cIgG+, sIgM-, cIgM-, sIgM-, sIg-kappa-, cIg-kappa-, sIg-lambda+, cIg-lambda+; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human flat-moded aneuploid hypertriploid karyotype - 79(72-79)<3n>XX, -X,-1, +3, +4, +5, +6, +8, +11, +12, -13, +14, +15, +16, +18, -19, +20, +21, +22, +dminx1-4, add(1)(p11-21), del(3)(q22), der(4)add(4)(p1?)t(4;11)(11;?)(q33;q13)(q24;?)x2, der(5)t(1;5)(p11;q35), del(6)(q15)x1-2, del(8)(p21)x1-2, add(9)(q34-35)x1-2, t(12;13)(p12;q11)x2 - matches published karyotype |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
