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Cell line: | M4A1-M2B9 |
DSMZ no.: | ACC 967 |
Species: | human (Homo sapiens) |
Cell type: | B cell precursor leukemia |
Origin: | established in 2020 from a patient-derived xenograft model (PDX) of a bone marrow sample from a 15-year-old boy with untreated B-cell acute lymphoblastic leukemia; cells carry a MEF2D::BCL9 fusion; transcriptome and exome data are available from the originators upon request (see reference) |
Reference(s): | 40741 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | single, round cells growing in suspension; image; image; image |
Medium: | 80-90% RPMI 1640 + 10-20% h.i. FBS + 1x Glutamax |
Subculture: | seed out at about 0.5 x 106 cells/ml; split
1:2 to 1:3 every 2-3 days; maintain between 0.3-0.5 x 106 cells/ml |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | about 48 hours |
Harvest: | maximal density of ca. 1.5 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in PCR assay |
Immunology: | CD3 -, CD10 +, CD13 -, CD19 +, CD20 -, CD34 -, CD37 -,
CD38 +, CD80 -, CD138 +, HLA-DR +, cy CD79a +, sm/cy IgG -,
sm/cy IgM +, sm/cy kappa -, sm/cy lambda +; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Molec. Genetics: | fusion gene MEF2D::BCL9 was verified by RT-PCR |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/-2 -, XMLV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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