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| Cell line: | M5-T1 |
| DSMZ no.: | ACC 11003 |
| Species: | rat (Rattus norvegicus) |
| Cell type: | mesothelioma |
| Origin: | established in 2009 from the peritoneal fluid of a 17-month-old male F344 rat dying from invasive malignant mesothelioma after 378 days of induction with crocidolite (blue asbestos) injected intraperitoneously. The cell line was described as neoplastic relative to the preneoplastic rat mesothelioma cell line F3-1 (ACC 11002). Described to form tumors in syngenic rats after orthotopic transplantation. |
| Reference(s): | 41053, 41273, 41274 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | sarcomatoid cells growing adherently as monolayer; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | seed out at ca. 1-2 x 106 cells/80 cm2; split confluent culture 1:3 to 1:6 every 2-4 days using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 24 hours |
| Harvest: | cell harvest of ca. 15-20 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species. |
| Viruses: | PCR: MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
