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Cell line: | MB-021 |
DSMZ no.: | ACC 85 |
Species: | insect - cabbage moth (Mamestra brassicae) (Mamestra brassicae) |
Cell type: | larval hemocytes |
Origin: | established from the larvae of the butterfly Mamestra brassicae (family Noctuidae, subfamily Noctuinae) (cabbage moth; Kohleule) |
Reference(s): | 14712 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | polymorphic round cells, partly adherent; image; image |
Medium: | 85% Grace's Insect Medium (Modified) + 15% h.i. FBS |
Subculture: | split confluent cultures 1:2 about twice a week, but with using trypsin (cells grow partly adherent, but can be detached without trypsin); maintain at room temperature (about 20 °C) without CO2 exposure ; confluent cultures can be stored at 4 °C for up to one week without loss of viability |
Incubation: | at 20 °C without CO2 (at room temperature, keep lid of flask closed) |
Harvest: | about 5-7 x 106 cells/80 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization assays |
Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mamestra brassicae species. |
Viruses: | PCR: SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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