| | |
|---|
| Cell line: | MB-1 |
| DSMZ no.: | ACC 638 |
| Species: | human (Homo sapiens) |
| Cell type: | anaplastic thyroid carcinoma |
| Origin: | established in 2007 from the large retrosternal mass resected before treatment from a 57-year-old man with rapidly fatal anaplastic thyroid cancer |
| Reference(s): | 14984 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | round epitheloid cells growing in monolayers; image; image |
| Medium: | 80% RPMI 1640 + 20% h.i. FBS + 2 mM L-glutamine |
| Subculture: | seed out at ca. 1-3 x 106 cells/175 cm2; optimal split ratio of 1:3 to 1:10 every 3-7 days using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 60-100 hours |
| Harvest: | cell harvest of ca. 10-15 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
