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| Cell line: | MC3T3-E1 |
| DSMZ no.: | ACC 210 |
| Species: | murine (mouse) (Mus musculus) |
| Cell type: | embryo/fetus calvaria fibroblasts |
| Origin: | established from the calvaria of an embryo/fetus C57BL/6 mouse; described in the literature to differentiate to osteoblasts and to produce collagen |
| Reference(s): | 14674 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent fibroblast-like cells growing as monolayer; image; image; image; image; image; image |
| Medium: | 80-90% alpha-MEM (with ribo- and deoxyribonucleosides) + 10-20% h.i. FBS + 2 mM L-glutamine |
| Subculture: | seed out at about 1-2 x 106 cells/80 cm2; split confluent culture 1:3 to 1:5 once or twice a week using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 1-2 days |
| Harvest: | cell harvest of ca. 6-7 x 106 cells/80 cm2 or 9 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Cytogenetics: | murine hypotetraploid karyotype with 11.5% polyploidy - 72(68-80)<4n> - centric fusion absent |
| Viruses: | ELISA: reverse transcriptase negative; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
