| |
---|
Cell line: | MDA-MB-231 |
DSMZ no.: | ACC 732 |
Species: | human (Homo sapiens) |
Cell type: | breast carcinoma |
Origin: | established in 1973 from the pleural effusion of a 51-year-old woman with invasive breast carcinoma after chemotherapy; widely used and extensively characterized cell line; triple-negative and described to be tumorigenic in nude mice; listed in the CCLE (ref 18232); a derivative expressing Cherry Fluorescent Protein is available (ACC 982); RNA sequence data are available (see Ref 40906 and RNA-Seq) |
Reference(s): | 14415, 17111, 17112, 18232, 18234, 40906 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| DSMZ Cell Culture Data: |
Morphology: | epithelial cells growing adherently as monolayer, sometimes forming spindle-like extensions; image; image; image |
Medium: | 90% Leibovitz´ L15 + 10% h.i. FBS |
Subculture: | seed out at ca. 0.5-1.0 x 106 cells/175 cm2; optimal split ratio 1:5 to 1:20 every 3-5 days using trypsin-EDTA |
Incubation: | at 37 °C without CO2 gas exchange (no filter lid, lid of flask closed) |
Doubling time: | ca. 25-30 hours |
Harvest: | cell harvest of ca. 10-20 x 106 cells/80 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in microbiological culture, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Molec. Genetics: | triple-negative confirmed by absence of ER, PR, AR and HER2 in Western blots |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
| Print data sheet |