| | |
|---|
| Cell line: | MDA-MB-231 |
| DSMZ no.: | ACC 732 |
| Species: | human (Homo sapiens) |
| Cell type: | breast carcinoma |
| Origin: | established in 1973 from the pleural effusion of a 51-year-old woman with invasive breast carcinoma after chemotherapy; widely used and extensively characterized cell line; triple-negative and described to be tumorigenic in nude mice; listed in the CCLE (ref 18232); derivatives expressing GFP (ACC 981) or Cherry Fluorescent Protein (ACC 982) are available; RNA sequence data are available (see Ref 40906 and RNA-Seq) |
| Reference(s): | 14415, 17111, 17112, 18232, 18234, 40906 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelial cells growing adherently as monolayer, sometimes forming spindle-like extensions; image; image; image; image; image; |
| Medium: | 90% Leibovitz´ L15 + 10% h.i. FBS |
| Subculture: | seed out at ca. 0.5-1.0 x 106 cells/175 cm2; optimal split ratio 1:5 to 1:20 every 3-5 days using trypsin-EDTA |
| Incubation: | at 37 °C without CO2 gas exchange (no filter lid, lid of flask closed) |
| Doubling time: | ca. 25-30 hours |
| Harvest: | cell harvest of ca. 10-20 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Molec. Genetics: | triple-negative confirmed by absence of ER, PR, AR and HER2 in Western blots |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
