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Cell line: | MDA-MB-231cherry |
DSMZ no.: | ACC 982 |
Species: | human (Homo sapiens) |
Cell type: | breast carcinoma |
Origin: | established in 2012 from the original MDA-MB-231 by transduction with lentiviral SIN vector expressing constitutively mcherry (original cell line established 1973 from the pleural effusion of a 51-year-old woman with breast carcinoma after chemotherapy; available at DSMZ ACC 732) |
Reference(s): | 40821, 40872 |
Biosafety level: | 1, GMO-S1 |
Risk assessment: | MDA-MB-231 cells were transduced with the lentiviral SIN vector pRRL.PPT.SF.mcherry.pre#. The transduction method is an approved third generation safety measure to prevent the formation of replication competent retroviruses. According to German legislation, the genetically modified cells are assigned to safety group S1. |
Permissions and restrictions: | A;D |
| DSMZ Cell Culture Data: |
Morphology: | epithelial cells growing adherently as monolayer; almost 100% cherry positive cells by Flow Cytometry; image; image; image |
Medium: | 90% Leibovitz´ L15 + 10% h.i. FBS |
Subculture: | seed out at ca. 0.5-1.0 x 106 cells in a T75 flask; split 1:5 to 1:20 every 3-5 days using trypsin-EDTA |
Incubation: | at 37 °C without CO2, keep lid closed |
Doubling time: | 24-30 hours |
Harvest: | cell harvest of 4-6 x 106 cells in a T75 flask |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in PCR assay |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database, identical to MDA-MB-231 |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV -, XMLV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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