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| Cell line: | ME-1 |
| DSMZ no.: | ACC 537 |
| Species: | human (Homo sapiens) |
| Cell type: | acute myeloid leukemia |
| Origin: | established from the peripheral blood of a 40-year-old man with acute myeloid leukemia (AML) FAB M4Eo at second relapse in 1988; cells were described in the literature to carry the inv(16)(p13q22) leading to the fusion gene CBFB-MYH11. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and RNA-Seq) |
| Reference(s): | 15038, 15071, 18187 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | round cells growing singly in suspension; image; image |
| Medium: | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture: | seed out at ca. 0.5-1.0 x 106 cells/ml in 24-well-plates; maintain at ca. 0.5-1.0 x 106 cells/ml; split saturated culture 1:2 every 3-5 days; minimum density at ca. 0.5 x 106 cells/ml; we recommend to cultivate cells allways in 12- or 24-well-plates |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 3-5 days |
| Harvest: | maximum cell density of ca. 1.0 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | CD3-, CD4+, CD13+, CD14-, CD15-, CD19-,
CD33+, CD34+, cyCD68; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hyperdiploid karyotype with 9% polyploidy; 48(45-48)<2n>XY, +8, +20, der(2)t(2;11)(q31;q13), der(9)t(9;18)(q12.2;q11), der(16)t(11;16)(q13;q22)inv(16)(p13q22), der(17)t(2;17)(p25;p12); sdl lacking trisomy 20; carries inversion/translocation effecting rearrangement of MYH11 and CBFB; resembles published karyotypes |
| Molec. Genetics: | expression of fusion gene CBFB-MYH11 confirmed by RT-PCR |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
