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| Cell line: | MEC-2 |
| DSMZ no.: | ACC 500 |
| Species: | human (Homo sapiens) |
| Cell type: | chronic B cell leukemia |
| Origin: | established in 1994 from the peripheral blood of a 62-year-old man with chronic B cell leukemia (B-CLL in prolymphocytoid transformation to B-PLL) prior to therapy; serial sister cell line of MEC-1 (DSM ACC 497) |
| Reference(s): | 14980 |
| Biosafety level: | 1 |
| Risk assessment: | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. According to a recommendation of the German Central Committee for Biological Safety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used in genetic engineering projects. |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | polymorphic cells growing in suspension in large clumps; clumps can easily be segregated; image; image; image |
| Medium: | 90% Iscove's MDM + 10% h.i. FBS |
| Subculture: | seed out at ca. 0.3-0.5 x 106 cells/ml; maintain at about 0.5 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 2-3 days; after thawing, during the first week cells should be kept with 20% FBS; starting the culture in 24-well-plate is of advantage; viability may not exceed 80% |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 40-50 hours |
| Harvest: | maximal density at about 1.0 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | CD3-, CD10-, CD13-, CD19+, CD20+, CD34-, CD37+, CD38(+), CD80+, CD138-, HLA-DR(+),
cyCD79a(+), cIgG-, cIgM+, cIg-kappa+, cIg-lambda-; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database, identical to sister cell line MEC-1 (ACC 497) |
| Cytogenetics: | human flat-moded hypertriploid karyotype with 7% polyploidy - 75-80<3n>XX/XXY, -6, -9, -10, +11, +12, -13, +15, -17, -21, +10mar - add(X)(p22)x2, inv(12)(?p12.2?q24.1)x2, add(18)(q2?1) - karyotypically distinct from MEC-1 (as reported) |
| Viruses: | PCR: EBV +, HBV -, HCV-1 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
