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Cell line: | MEG-01 |
DSMZ no.: | ACC 364 |
Species: | human (Homo sapiens) |
Cell type: | chronic myeloid leukemia in megakaryocytic blast crisis |
Origin: | established from the bone marrow of a 55-year-old man with chronic myeloid leukemia (CML) in megakaryocytic blast crisis in 1983; cells are described in the literature to carry t(9;22) leading to BCR-ABL1 e13-a2 (b2-a2) fusion gene |
Reference(s): |
14812, 14813, 14479, 14488 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | growing singly or in small clusters in suspension, some cells slightly adherent (with an epithelial-like morphology); image; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS |
Subculture: | seed out at ca. 0.3 - 0.5 x 106 cells/ml; split saturated culture 1:3 to 1:4 every 3 days |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 35 hours |
Harvest: | maximal density at ca. 1.3 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | CD3 -, CD13 +, CD15 +, CD19 -, CD33 +; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hyperdiploid karyotype with 12% polyploidy - 54(53-56)<2n>XY, +6, +19, +19, +21, +3-4mar, t(1;15)(p13;p13), ?inv(3)(p25q26), i(4q), add(5)(p15), der(9)t(9;22)(q34;q11)x2, add(10)(p14), dup(13)(q13q33-34), add(14)(p11), der(22)t(9;22)(q34;q11) - r/dmin x1 present at 57% - sideline with i(5)(q10)del(5)(q11q13) instead of add(5) - resembles published karyotype |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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