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Cell line: | MEGAL |
DSMZ no.: | ACC 719 |
Species: | human (Homo sapiens) |
Cell type: | acute megakaryoblastic leukemia |
Origin: | derived from a case of acute megakaryocytic leukemia (AML M7); cell line expresses the SET-NUP214 fusion transcript. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and RNA-Seq) |
Reference(s): | 16550, 18187 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | small round cells, growing singly in suspension; image; image; image; image |
Medium: | 80% RPMI 1640 + 20% h.i. FBS |
Subculture: | seed out at ca. 1 x 106 cells/ml in 24-well plate; cells may grow slowly in the first week after thawing; split culture 1:2 twice a week; cells are difficult to culture |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 70 hours |
Harvest: | maximum density at ca. 2 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in microbiological culture, PCR assays |
Immunology: | CD3 -, CD13 -, CD14 -, CD19 -, CD33 +, CD34 +, CD71 +, CD235a -; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hyperdiploid karyotype; 49(45-52)<2n>XX, -8, +13, +13, -15, -16, -17, +5 mar, del(2)(q11q22), t(2;15)(p16;q14), der(6)del(6)(p23)t(6;17)(q27;q11), del(9)(q34.11q34.13), ins(13;16)(q31;q13q23), ?r(13)(q31q32)x3, der(14)t(13;14)(p12;q12)t(14;9)(q21;q11), del(16)(q13q23), der(19)t(15;19)(q?;p13); karyotype hitherto unreported; carries cryptic 9q34 deletion effecting SET-NUP214 fusion; rearrangements at 13q31 associated with activation of miR-17~92 cluster |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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