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| Cell line: | MEL-745A cl. DS19 |
| DSMZ no.: | ACC 501 |
| Species: | murine (mouse) (Mus musculus) |
| Cell type: | erythroleukemia |
| Origin: | Mouse erythroleukemia (MEL) cells were transformed by Friend leukemia virus strain 745A; the clone DS19 is described in the literature to be DMSO sensitive, resulting in erythroid differentiation (please note that DSMZ has not confirmed differentiation potential and does not guarantee a successful differentiation) |
| Reference(s): | 14545, 14816, 14965 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | round, single or in small clusters, growing in suspension, about 5% giant cells; image; image; image |
| Medium: | 80-90% DMEM + 10-20% h.i. FBS + 2 mM L-glutamine |
| Subculture: | seed out at ca. 5-10 x 104 cells/ml; split saturated culture 1:8 to 1:10 every 3 days; maintain at about 0.05-0.5 x 106 cells/ml; during continuous culture, the cell density should not be higher than 0.5 x 106 cells/ml |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 20 hours |
| Harvest: | maximal density at about 0.8 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Cytogenetics: | murine hypertetraploid karyotype |
| Viruses: | PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
