| |
---|
Cell line: | MFE-280 |
DSMZ no.: | ACC 410 |
Species: | human (Homo sapiens) |
Cell type: | endometrium adenocarcinoma |
Origin: | established from tumor tissue from a 77-year-old woman with recurrence of endometrial carcinoma (adenomatous, partly papillary, grade G3) in 1990; described as forming heterotransplantable tumors in nude mice and as carrying receptors for progesterone |
Reference(s): | 14587, 14588 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | epitheloid cells growing adherently in monolayers; image; image; image; image; image |
Medium: | 45% RPMI 1640 + 45% MEM (with Earle's salts) + 10% h.i. FBS + 2 mM L-glutamine + 1x insulin-transferrin-sodium selenite |
Subculture: | seed out at 2-6 x 106 cells/75 cm2; about 4 hours after thawing, floating cells and medium should be decanted into a second flask; split confluent culture 1:2 to 1:6 every 3-5 days using trypsin/EDTA |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 60-90 hours |
Harvest: | cell harvest of ca. 20 x 106 cells/75 cm2 flask |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hyperdiploid karyotype with 24% polyploidy - 52(48-52)<2n>X, -X, +1, +1, -2, +9, -10, -12, -13, -16, -17, -17, -18, -19, -20, -22, +18mar, add(1)(p34), add(1)(p3?), add(1)(q11), add(3)(q13), add(3)(q27-29), add(4)(p14), der(9)t(9;13)(p11;q11), add(15)(p11) - near diploidy and gain of 1q typical in endometrial carcinoma |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
| Print data sheet |