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| Cell line: | MH1C1 |
| DSMZ no.: | ACC 106 |
| Species: | rat (Rattus norvegicus) |
| Cell type: | hepatoma |
| Origin: | a clonal strain of epithelial cells was derived from a transplantable Morris hepatoma (#7795, Buffalo strain) in 1967; cells were described in the literature to synthesize and to secrete serum albumin and serum complement component C9 |
| Reference(s): | 14531, 14890, 14930, 15209 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent cells growing in big clusters, different cellular forms; image; image |
| Medium: | 85-90% DMEM + 10-15% h.i. FBS |
| Subculture: | split confluent culture 1:2 to 1:4 about once or twice per week using trypsin/EDTA; seed out at ca. 2 x 106 cells/75 cm2 |
| Incubation: | at 37 °C with 10% CO2 |
| Doubling time: | ca. 33-60 hours |
| Harvest: | cell harvest of about 20 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species. |
| Cytogenetics: | near-diploid rat karyotype with 10% polyploidy - 44(40-46)<2n> |
| Viruses: | ELISA: reverse transcriptase negative; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
