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single, relatively small round cells growing in suspension; image; image
80% RPMI 1640 + 20% h.i. FBS
cells may be difficult to culture; seed out at ca. 1 x 106 cells/ml; cells grow best in 24-well-plates; during the first 2-6 days proliferation rate may be rather low but the medium rarely becomes acidic (yellow), however it is advisable to change the spent medium every 2-3 days; split saturated culture 1:2; minimum density at 0.8 x 106 cells/ml, maximum density at ca. 5 x 106 cells/ml but optimal density at ca. 1-2 x 106 cells/ml
at 37 °C with 5% CO2
about 120 hours
cell harvest of ca. 2 x 106 cells/ml in a 24-well-plate
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
human pseudodiploid karyotype - 46(45-46)<2n>XX, del(6)(q15), der(9)t(9;9)(p21;q11), der(19)t(1;19)(q23;p13) - carries t(1;19) primary and 6q- secondary rearrangements associated with pre B-ALL - resembles published karyotype