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| Cell line: | MHH-CALL-3 |
| DSMZ no.: | ACC 339 |
| Species: | human (Homo sapiens) |
| Cell type: | B cell precursor leukemia |
| Origin: | established from the bone marrow of an 11-year-old girl with pre B cell acute lymphoblastic leukemia (ALL) in 1993 (at diagnosis) |
| Reference(s): | 15018 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | single, relatively small round cells growing in suspension; image; image |
| Medium: | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture: | cells may be difficult to culture; seed out at ca. 1 x 106 cells/ml; cells grow best in 24-well-plates; during the first 2-6 days proliferation rate may be rather low but the medium rarely becomes acidic (yellow), however it is advisable to change the spent medium every 2-3 days; split saturated culture 1:2; minimum density at 0.8 x 106 cells/ml, maximum density at ca. 5 x 106 cells/ml but optimal density at ca. 1-2 x 106 cells/ml |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | about 120 hours |
| Harvest: | cell harvest of ca. 2 x 106 cells/ml in a 24-well-plate |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD38 +, cyCD79a +, CD80 -, CD138 +, HLA-DR +; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human pseudodiploid karyotype - 46(45-46)<2n>XX, del(6)(q15), der(9)t(9;9)(p21;q11), der(19)t(1;19)(q23;p13) - carries t(1;19) primary and 6q- secondary rearrangements associated with pre B-ALL - resembles published karyotype |
| Molec. Genetics: | fusion gene TCF3::PBX1 was verified by RT-PCR |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
